N-Acetyll-β-D-Glucosaminidase Activities in Urine of Cadmium Workers
نویسندگان
چکیده
منابع مشابه
Urinary Activities of N-acetyl-β-D-glucosaminidase and Its Isoenzyme B in Cadmium-Exposed Rats
This paper aims to assess the relationship between the urinary activity of N-acetyl-β-D-glucosaminidase (NAG) and its isoenzyme B (NAG-B) and cadmium (Cd) concentration in the urine as well as to evaluate which of these lysosomal enzymes may be a more useful biomarker for the monitoring of Cd-induced tubular damage. For this purpose we have used an experimental model in rats chronically exposed...
متن کاملUrinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A & B in workers exposed to cadmium at cadmium plating
OBJECTIVE The present study was carried out to determine the effect of cadmium exposure on Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B in workers exposed at cadmium plating. METHODS 50 subjects using cadmium during cadmium plating formed the study group. An equal number of age-sex matched subjects working in administrative section formed the control group. Urinary cadm...
متن کاملEstimation and separation of N-acetyl-beta-D-glucosaminidase isoenzymes in urine.
N-acetyl-beta-D-glucosaminidase is a lysosomal enzyme made up of two isoenzymes (A and B). It has been used extensively as a marker for kidney damage. However, its estimation in urine has not been standardized. We have established a method for the estimation and separation of NAG isoenzymes by ion-exchange chromatography. In 19 experiments done so far, this method has given reproducible results...
متن کاملRapid assay of N-acetyl-beta-D-glucosaminidase isoenzymes in urine by ion-exchange chromatography.
We describe a new method for separating and measuring urinary N-acetyl-beta-D-glucosaminidase isoenzymes by "high-performance" liquid chromatography. Isoenzymes are eluted from the anion-exchange column with a one-step linear gradient of NaCl solution. For continuous post-column quantification of the activities of these isoenzymes, we use an on-line post-column reactor and 4-methylumbelliferyl-...
متن کاملN-Acetyl-P-D-glucosaminidase in Marmoset Kidney, Serum and Urine
N-Acetyl-fi-D-glucosaminidase activities were determined in homogenates of marmoset kidney, in serum and in urine by using the 4-methylumbelliferyl substrate. The enzyme activity was separated into several components by DEAE-cellulose ion-exchange chromatography, starch-gel electrophoresis and isoelectric focusing. The kidney contained two major forms of the enzyme, A and B, which had similar p...
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ژورنال
عنوان ژورنال: Sangyo Igaku
سال: 1982
ISSN: 0047-1879,1881-1302
DOI: 10.1539/joh1959.24.7_858_2